Coding

Part:BBa_K4161506:Design

Designed by: Lingrui Lai   Group: iGEM22_DKU   (2022-07-26)


332aa, ipaD protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 708


Design Notes

This sequence is designed to be expressed in eukaryotes as we select S. cerevisiae as our engineered organisms. Therefore, this sequence should use eukaryote codons instead of prokaryote ones.


Source

Shigella flexneri

References